phic31 integrase (Addgene inc)
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Phic31 Integrase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phic31+integrase/T4+Lysozyme+A82P+(Plasmid+%2318396)/pmc12343897-155-45-47
Average 93 stars, based on 6 article reviews
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Quantitative RT-PCR:Article Title: Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals Chloramphenicol Sigma C0378 Spectinomycin DOT Scientific DSS23000–5 Kanamycin American Bio AB01100 Apramycin Fisher AAJ66616 Carbenicillin Sigma C1389 G418 Fisher 10131035 Hygromycin B Fisher 10687010 Luria Broth American Bio AB01198 M9 Minimal Media Fisher DF0485–17 Erythromycin Acros 227330050 Amberlite XAD-7 Resin Acros 202245000 Celite Acros 349675000 Critical Commercial Assays Luna Universal qRT-PCR kit NEB E3005 Purexpress translation kit NEB E6800S HiScribe T7 RNA Kit NEB E2040S Experimental Models: Organisms and Strains See Extended Data S2 for detailed list Indicated when externally acquired Recombinant DNA Source of Recombinant:Article Title: Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals Chloramphenicol Sigma C0378 Spectinomycin DOT Scientific DSS23000–5 Kanamycin American Bio AB01100 Apramycin Fisher AAJ66616 Carbenicillin Sigma C1389 G418 Fisher 10131035 Hygromycin B Fisher 10687010 Luria Broth American Bio AB01198 M9 Minimal Media Fisher DF0485–17 Erythromycin Acros 227330050 Amberlite XAD-7 Resin Acros 202245000 Celite Acros 349675000 Critical Commercial Assays Luna Universal qRT-PCR kit NEB E3005 Purexpress translation kit NEB E6800S HiScribe T7 RNA Kit NEB E2040S Experimental Models: Organisms and Strains See Extended Data S2 for detailed list Indicated when externally acquired Recombinant DNA Source of Construct:Article Title: Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals Chloramphenicol Sigma C0378 Spectinomycin DOT Scientific DSS23000–5 Kanamycin American Bio AB01100 Apramycin Fisher AAJ66616 Carbenicillin Sigma C1389 G418 Fisher 10131035 Hygromycin B Fisher 10687010 Luria Broth American Bio AB01198 M9 Minimal Media Fisher DF0485–17 Erythromycin Acros 227330050 Amberlite XAD-7 Resin Acros 202245000 Celite Acros 349675000 Critical Commercial Assays Luna Universal qRT-PCR kit NEB E3005 Purexpress translation kit NEB E6800S HiScribe T7 RNA Kit NEB E2040S Experimental Models: Organisms and Strains See Extended Data S2 for detailed list Indicated when externally acquired Recombinant DNA Source of Software:Article Title: Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals Chloramphenicol Sigma C0378 Spectinomycin DOT Scientific DSS23000–5 Kanamycin American Bio AB01100 Apramycin Fisher AAJ66616 Carbenicillin Sigma C1389 G418 Fisher 10131035 Hygromycin B Fisher 10687010 Luria Broth American Bio AB01198 M9 Minimal Media Fisher DF0485–17 Erythromycin Acros 227330050 Amberlite XAD-7 Resin Acros 202245000 Celite Acros 349675000 Critical Commercial Assays Luna Universal qRT-PCR kit NEB E3005 Purexpress translation kit NEB E6800S HiScribe T7 RNA Kit NEB E2040S Experimental Models: Organisms and Strains See Extended Data S2 for detailed list Indicated when externally acquired Recombinant DNA Source of Membrane:Article Title: Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 variants Article Snippet: .. Freshly laid eggs were aligned along a wet nitrocellulose membrane as previously described ( Volohonsky et al . 2015 ) and injected with 400 ng/μl DNA in 0.5× phosphate-buffered saline (PBS) in the posterior pole, using quartz microcapillaries: 300 ng/μl transgenesis plasmid and 100 ng/μl helper plasmid encoding either piggyBac transposase ( Lutrat et al . 2023 ) or Article Title: Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 promoter variants Article Snippet: .. Freshly laid eggs were aligned along a wet nitrocellulose membrane as previously described and injected with 400 ng/μl DNA in 0.5× phosphate-buffered saline (PBS) in the posterior pole, using quartz microcapillaries: 300 ng/μl transgenesis plasmid and 100 ng/μl helper plasmid encoding either piggyBac transposase or Injection:Article Title: Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 variants Article Snippet: .. Freshly laid eggs were aligned along a wet nitrocellulose membrane as previously described ( Volohonsky et al . 2015 ) and injected with 400 ng/μl DNA in 0.5× phosphate-buffered saline (PBS) in the posterior pole, using quartz microcapillaries: 300 ng/μl transgenesis plasmid and 100 ng/μl helper plasmid encoding either piggyBac transposase ( Lutrat et al . 2023 ) or Article Title: Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 promoter variants Article Snippet: .. Freshly laid eggs were aligned along a wet nitrocellulose membrane as previously described and injected with 400 ng/μl DNA in 0.5× phosphate-buffered saline (PBS) in the posterior pole, using quartz microcapillaries: 300 ng/μl transgenesis plasmid and 100 ng/μl helper plasmid encoding either piggyBac transposase or Saline:Article Title: Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 variants Article Snippet: .. Freshly laid eggs were aligned along a wet nitrocellulose membrane as previously described ( Volohonsky et al . 2015 ) and injected with 400 ng/μl DNA in 0.5× phosphate-buffered saline (PBS) in the posterior pole, using quartz microcapillaries: 300 ng/μl transgenesis plasmid and 100 ng/μl helper plasmid encoding either piggyBac transposase ( Lutrat et al . 2023 ) or Article Title: Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 promoter variants Article Snippet: .. Freshly laid eggs were aligned along a wet nitrocellulose membrane as previously described and injected with 400 ng/μl DNA in 0.5× phosphate-buffered saline (PBS) in the posterior pole, using quartz microcapillaries: 300 ng/μl transgenesis plasmid and 100 ng/μl helper plasmid encoding either piggyBac transposase or Plasmid Preparation:Article Title: Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 variants Article Snippet: .. Freshly laid eggs were aligned along a wet nitrocellulose membrane as previously described ( Volohonsky et al . 2015 ) and injected with 400 ng/μl DNA in 0.5× phosphate-buffered saline (PBS) in the posterior pole, using quartz microcapillaries: 300 ng/μl transgenesis plasmid and 100 ng/μl helper plasmid encoding either piggyBac transposase ( Lutrat et al . 2023 ) or Article Title: Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 promoter variants Article Snippet: .. Freshly laid eggs were aligned along a wet nitrocellulose membrane as previously described and injected with 400 ng/μl DNA in 0.5× phosphate-buffered saline (PBS) in the posterior pole, using quartz microcapillaries: 300 ng/μl transgenesis plasmid and 100 ng/μl helper plasmid encoding either piggyBac transposase or other:Article Title: Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome Article Snippet: As internal reference genes for normalization, for S. cerevisiae UBC6 was used, and for E. coli cysG was used. |
